What is immunoassay nedir?

Immunoassay is a powerful technique used in modern biomedical research and clinical practice to quantitatively detect and measure specific target molecules or substances in various biological samples. It is a highly sensitive and specific analytical method that relies on the molecular recognition and binding interactions between an antibody and its corresponding antigen.

Immunoassay is widely used for the detection of proteins, hormones, drugs, and various other molecules of clinical and research importance. The most commonly used immunoassay techniques include enzyme-linked immunosorbent assay (ELISA), radioimmunoassay (RIA), and fluorescent immunoassay (FIA).

The immunoassay process involves several key steps, including sample preparation, binding of the target molecule to an immobilized antibody, washing away unbound materials, detection of the bound complex, and quantification of the results. The detection methods can vary depending on the assay type and can include colorimetric, luminescent, or fluorescent readouts.

Immunoassays are widely used in various fields of biomedical research, including molecular biology, clinical diagnostics, drug discovery, and environmental and food safety testing. They offer high sensitivity, specificity, and precision, making them valuable tools for detecting and measuring low-abundance biomolecules in complex biological matrices.